Homogeneous Immunoassay

Lumigen SPARCL is a homogeneous proximity assay technology utilizing flash chemiluminescence detection without solid support or wash steps allowing for assays to be completed in less than 30 minutes. The SPARCL technology enables assays to be miniaturized for high throughput screening while maintaining sensitive results with good dynamic range. SPARCL can be automated and adapted for multiple applications including ELISA, protein-protein and protein-nucleic acid interactions, and high throughput binding assays.

• Complete assays in less than 30 minutes - Save time with only one incubation step, no separation or wash steps and instantaneous signal generation from flash chemiluminescence
• Cost effective - Use fewer reagents and instruments with no need for plate/bead coating operations, automated washing stations or other expensive detection equipment.
• Flexible assay formats - Adaptable for many different kinds of assays in solution or solid phase formats to study a wide variety of targets
• Produce less waste - No wash means no disposal of wash solution. SPARCL produces only 1x well volume of waste compared to 13x well volume for conventional ELISA assays.

SPARCL Formats

Solution or solid phase assay
SPARCL can be implemented in formats with or without a solid phase. When using a solid phase, both the acridan compound and a specific capture antibody are coupled to solid phases such as micro particles or microtiter plates, whereas when the solid phase is omitted the capture antibody is directly labeled with the acridan compound. The solution phase format eliminates the need for plate or particle coating, improves kinetics to shorten incubation time and due to the lack of a solid/solution interface offers a more native biological environment.

SPARCL Technology

SPARCL (Spatial Proximity Analyte Reagent Capture Luminescence) is a proximity dependent chemiluminescent technology for the detection of specific binding interaction or association between two binding partners. In a SPARCL assay, a binding partner labeled with a chemiluminescent substrate (acridan) and a second binding partner labeled with horseradish peroxidase (HRP) are brought into close proximity to each other through a specific binding event. Because of this close proximity of acridan to the HRP enzyme a flash of chemiluminescence is generated upon addition of a trigger solution containing hydrogen peroxide and an enhancer.